小鼠FGF-21 ELISA试剂盒(ab212160)
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| 产品名称 | 小鼠FGF-21 ELISA试剂盒 |
|---|---|
| 品牌 | 艾博抗Abcam |
| 货号 | ab212160 |
| 精确度 | 批次内 样品 n Mean SD CV% Serum 5 3% 批次间 样品 n Mean SD CV% Serum 3 4% |
| 样品类型 | Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma |
| 灵敏度 | 1.47 pg/ml |
| 范围 | 9.375 pg/ml - 600 pg/ml |
| 回收率 | 特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 94 91% - 97% Serum 103 99% - 107% Hep Plasma 94 88% - 104% EDTA Plasma 100 92% - 108% Cit plasma 101 94% - 106% |
| 检测时间 | 1h 30m |
| 种属反应性 | 与反应: Mouse不与反应: Cow |
| 产品概述 | Mouse FGF-21 ELISA Kit (ab212160) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FGF-21 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse FGF-21 with 1.47 pg/ml sensitivity.SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits: - Single-wash protocol reduces assay time to 90 minutes or less - High sensitivity, specificity and reproducibility from superior antibodies - Fully validated in biological samples - 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits. ASSAY SPECIFICITY This kit recognizes both native and recombinant mouse FGF-21 protein in serum, plasma, and cell culture supernatant samples only.INTERFERENCE Recombinant mouse FGF R3 and mouse FGF R4 were prepared at 50 ng/mL and 1 ng/mL and tested for interference. No interference with was observed.SPECIES REACTIVITY This kit recognizes mouse FGF-21 protein. Rat serum and plasma samples are not compatible with this kit. Other species reactivity was determined by measuring 100% serum samples of various species, interpolating the protein concentrations from the mouse standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in mouse serum assayed at the same dilution. Reactivity < 3% was determined for the following species: Human and cow. |
| 研究领域 | Cardiovascular Angiogenesis Growth Factors FGF FGF Signal Transduction Growth Factors/Hormones FGF Cancer Growth factors FGF |
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